Binding induced strand displacement amplification for homogeneous protein assay

作者：Li, Yubin^*; Liu, Sheng; Zhao, Zike; Zheng, Yuner; Wang, Zirui

来源：Talanta, 2017, 164: 196-200.

DOI：10.1016/j.talanta.2016.11.047

• Protein Platelet-derived growth factor
• Binding-induced strand displacement amplification
• Homogeneous assay

An ultrasensitive and homogenous strategy for protein assay was established based upon binding-induced strand displacement amplification (BI-SDA). Binding-Induced DNA strand-displacement occurred between Apt-T center dot signal DNA and Apt-C, and release of signal DNA upon addition of platelet-derived growth factor (PDGF BB). The released signal DNA further hybridized with multifunctional hairpin DNA probe and induced the strand-displacement amplification in the presence of Klenow Fragment (exo(-)) and dNTPs. The BI-SDA product contain G-quaruplex DNA, which could be recognized and reported by the fluorescence of fluorochrome N methyl porphyrin propionic acid IX (NMM). The fluorescence intensity was proportional to the concentration of PDGF-BB over the range of 1.0x10(-11) mol/L -2.0x10(-9) mol/L, with a detection limit of 3.6 pmol/L. This proposed strategy showed good selectivity and practicality, and might be applied to other proteins in the future.

• 出版日期2017-3-1
• 单位广东海洋大学