A high-throughput, image-based screen to identify kinases involved in brown adipocyte development

作者：Perdikari Aliki; Kulenkampff Elisabeth; Rudigier Carla; Neubauer Heike; Luippold Gerd; Redemann Norbert; Wolfrum Christian^*

来源：Science Signaling, 2017, 10(466): eaaf5357.

DOI：10.1126/scisignal.aaf5357

Brown adipose tissue (BAT) is responsible for thermogenesis that is not associated with shivering through the process of converting chemical energy into heat through uncoupling protein 1 (UCP1) in the mitochondria. Thus, expanding or activating BAT could be a potential tool against obesity. To analyze the effect of kinase signaling on brown adipocyte formation, a process that describes the acquisition of the ability to dissipate energy as heat, we performed lentiviral-mediated short hairpin knockdown or used pharmacological inhibitors in a high-content and high-throughput in vitro image-based screen. We identified 190 kinases that either stimulated or inhibited brown adipocyte proliferation, differentiation, or formation. Among these kinases, we found that 5' AMP-activated protein kinase (AMPK) promoted the formation of brown adipocytes abundant in UCP1. Together, our results provide insight into the kinases, particularly AMPK, that regulate brown adipocyte formation.

• 出版日期2017-2-14