Development of an Indirect ELISA for Serological Diagnosis of Bovine herpesvirus 5

作者：Dummer Luana A; Araujo Itaua L; Campos Fabricio S; da Rosa Matheus C; Finger Paula F; de Oliveira Patricia D; Conceicao Fabricio R; Fischer Geferson; Roehe Paulo M; Leite Fabio P L^*

来源：PLos One, 2016, 11(2): e0149134.

DOI：10.1371/journal.pone.0149134

Bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5) are economically important pathogens, associated with a variety of clinical syndromes, including respiratory and genital disease, reproductive failure and meningoencephalitis. The standard serological assay to diagnose BoHV-1 and BoHV-5 infections is the virus neutralization test (VNT), a time consuming procedure that requires manipulation of infectious virus. In the present study a highly sensitive and specific single dilution indirect ELISA was developed using recombinant glycoprotein D from BoHV-5 as antigen (rgD5ELISA). Bovine serum samples (n = 450) were screened by VNT against BoHV-5a and by rgD5ELISA. Compared with the VNT, the rgD5ELISA demonstrated accuracy of 99.8%, with 100% sensitivity, 96.7% specificity and coefficient of agreement between the tests of 0.954. The rgD5ELISA described here shows excellent agreement with the VNT and is shown to be a simple, convenient, specific and highly sensitive virus-free assay for detection of serum antibodies to BoHV-5.

• 出版日期2016-2-11