Reprogramming of tRNA modifications controls the oxidative stress response by codon-biased translation of proteins

作者：Chan Clement T Y; Pang Yan Ling Joy; Deng Wenjun; Babu I Ramesh; Dyavaiah Madhu; Begley Thomas J; Dedon Peter C^*

来源：Nature Communications, 2012, 3(1): 937.

DOI：10.1038/ncomms1938

Selective translation of survival proteins is an important facet of the cellular stress response. We recently demonstrated that this translational control involves a stress-specific reprogramming of modified ribonucleosides in tRNA. Here we report the discovery of a step-wise translational control mechanism responsible for survival following oxidative stress. In yeast exposed to hydrogen peroxide, there is a Trm4 methyltransferase-dependent increase in the proportion of tRNA(Leu((C) under bar AA)) containing m(5)C at the wobble position, which causes selective translation of mRNA from genes enriched in the TTG codon. Of these genes, oxidative stress increases protein expression from the TTG-enriched ribosomal protein gene RPL22A, but not its unenriched paralogue. Loss of either TRM4 or RPL22A confers hypersensitivity to oxidative stress. Proteomic analysis reveals that oxidative stress causes a significant translational bias towards proteins coded by TTG-enriched genes. These results point to stress-induced reprogramming of tRNA modifications and consequential reprogramming of ribosomes in translational control of cell survival.

• 出版日期2012-7