Microbial production of isoquinoline alkaloids as plant secondary metabolites based on metabolic engineering research

作者：Sato Fumihiko^*; Kumagai Hidehiko

来源：Proceedings of the Japan Academy Series B-Physical and Biological Sciences, 2013, 89(5): 165-182.

DOI：10.2183/pjab.89.165

• aromatic amino acid metabolism
• isoquinoline alkaloid biosynthesis
• metabolic engineering
• microbial production

Plants produce a variety of secondary metabolites that possess strong physiological activities. Unfortunately, however, their production can suffer from a variety of serious problems, including low levels of productivity and heterogeneous quality, as well as difficulty in raw material supply. In contrast, microorganisms can be used to produce their primary and some of their secondary metabolites in a controlled environment, thus assuring high levels of efficiency and uniform quality. In an attempt to overcome the problems associated with secondary metabolite production in plants, we developed a microbial platform for the production of plant isoquinoline alkaloids involving the unification of the microbial and plant metabolic pathways into a single system. The potential applications of this system have also been discussed.

• 出版日期2013-5