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摘要
Mutation breeding has been used to develop numerous plant varieties in recent decades. This paper describes an investigation into the scope for using gamma irradiation to create genetic variation in three M-2 generation Iranian rice cultivars (Sange-tarom, Tarom-hashemi and Nemat), using intron-exon splice junctions (ISJ) and analysis of morphological traits to identify mutants. The M-2 genotypes were screened using 21 semi-random primers and 10 morphological traits. Of the 165 bands amplified by the semi-random primers, 67% were polymorphic. The average number of bands per primer and genotype were 12.3 and 11.0, respectively. The size of the amplified fragments ranged from 250 bp to 1.8 kbp. The most informative ISJ primers were IT31-15 and IT35-15. The genetic similarity between control and mutant plants ranged from 6 to 93%, 36.4 to 96.8% and 17.4 to 95% in the Nemat, Sange-tarom and Tarom-hashemi M-2 generations, respectively. Cluster analysis based on the Dice similarity coefficient using the UPGMA procedure grouped the mutant genotypes into four clusters. Morphological analyses of 97 selected genotypes with a maturation time of between 105 and 135 days and heights of 45 to 160 cm showed that there was enough variation to create promising mutant lines for breeding programs. The results obtained using the ISJ primers were consistent with those based on morphological analysis and have considerable potential for detecting mutants.
- 出版日期2011-12
