ALTERED INTRACELLULAR TARGETING PROPERTIES ASSOCIATED WITH MUTATIONS IN THE LEGIONELLA-PNEUMOPHILA DOTA GENE

作者：BERGER KH; MERRIAM JJ; ISBERG RR

来源：Molecular Microbiology, 1994, 14(4): 809-822.

DOI：10.1111/j.1365-2958.1994.tb01317.x

Legionella pneumophila dot mutations cause defects in intracellular targeting of the microorganism within cultured macrophages. Each of the previously characterized dot mutations was shown to be complemented by a single open reading frame designated dotA. The defects caused by the mutations appear to be due to disrupted function of the predicted 1048-amino-acid residue DotA protein, and not by polarity effects on a downstream gene. Complementation studies indicated that the product of the dotA53 mutation results in a partially functional DotA protein, consistent with a stable N-terminal fragment having biological activity.

• 出版日期1994-11