Molecular Cloning of alpha RYR Hotspot Region 1 from Broiler Chicken

作者：Ziober Iris Lamberti; Paiao Fernanda Gonzales; Rockenbach Marin Silvana Regina; Marchi Denis Fabricio; Binneck Eliseu; Nepomuceno Alexandre Lima; Coutinho Luiz Lehmann; Shimokomaki Massami^*

来源：Brazilian Archives of Biology and Technology, 2009, 52(spe): 225-231.

DOI：10.1590/s1516-89132009000700029

• alpha RYR
• PSE
• chicken

Samples of Pectoralis major m. were collected, and an RT-PCR analysis of the alpha-Ryanodine receptor (alpha RYR) from chicken mRNA hotspot region spanning aminoacid residues 386 to 540, numbered according to the turkey sequence, revealed two classes of transcripts. The sequences of the first class were similar to turkey and human with 97% and 74% of identity, respectively, and included all transcripts with substitutions in the nucleotide sequence. The second class was characterized by the deletion of nucleotides, leading to a premature stop codon and coding for a truncated and nonfunctional protein. These results are to date the first report related to the sequencing of the chicken alpha RYR hotspot region 1, which will possibility serve as a guide for further studies regarding a solution in the poultry production chain related to the problem of pale, soft and exudative (PSE) meat.

• 出版日期2009-11