A Mutant H3N2 Influenza Virus Uses an Alternative Activation Mechanism in TMPRSS2 Knockout Mice by Loss of an Oligosaccharide in the Hemagglutinin Stalk Region

作者：Sakai Kouji^*; Sekizuka Tsuyoshi; Ami Yasushi; Nakajima Noriko; Kitazawa Minori; Sato Yuko; Nakajima Katsuhiro; Anraku Masaki; Kubota Toru; Komase Katsuhiro; Takehara Kazuaki; Hasegawa Hideki; Odagiri Takato; Tashiro Masato; Kuroda Makoto; Takeda Makoto

来源：Journal of Virology, 2015, 89(9): 5154-5158.

DOI：10.1128/JVI.00124-15

The host protease TMPRSS2 plays an essential role in proteolytic activation of the influenza A virus (IAV) hemagglutinin (HA) protein possessing a monobasic cleavage site. However, after passages in TMPRSS2 knockout mice, an H3N2 subtype IAV began to undergo cleavage activation of HA, showing high virulence in the mice due to the loss of an oligosaccharide at position 8 in the HA stalk region. Thus, the H3N2 IAV acquired cleavability by an alternative HA activation mechanism/protease(s).

• 出版日期2015-5