Determination of steroids adulterated in liquid herbal medicines using QuEChERS sample preparation and high-performance liquid chromatography

作者：Klinsunthorn Nantana; Petsom Amorn; Nhujak Thumnoon^*

来源：Journal of Pharmaceutical and Biomedical Analysis, 2011, 55(5): 1175-1178.

DOI：10.1016/j.jpba.2011.03.046

• Dispersive-solid phase extraction
• Herbal medicines
• HPLC
• QuEChERS
• Steroids

QuEChERS sample preparation was optimized using solvent extraction with acetonitrile and dispersive-solid phase extraction with primary and secondary amine sorbents, and validated for high-performance liquid chromatographic determination of nine steroids commonly used to adulterate herbal medicines: such as triamcinolone, prednisolone, hydrocortisone, methylprednisolone, betamethasone, dexamethasone, beclomethasone, fludrocortsone acetate and cortisone acetate. Satisfactory extraction recoveries of 91-113% for all nine steroids were obtained, along with an acceptable precision in extraction recoveries shown by R.S.D. of <= 4.6 and 3.2% for intraday and interday, respectively. The QuEChERS sample preparation developed here allows the reliable detection of adulterated steroids with the limits of detection in the range of 0.06-0.17 ppm. Adulterated steroids in three out of six real commercial liquid herbal medicines were found, such as 1.6 and 8.8 ppm dexamethasone and 0.43 ppm prednisolone.

• 出版日期2011-7-15