AN OPEN READING FRAME IN THE APPROXIMATELY 28-KB TOX-ARGK GENE-CLUSTER ENCODES A POLYPEPTIDE WITH HOMOLOGY TO FATTY-ACID DESATURASES

作者：HATZILOUKAS E; PANOPOULOS NJ; DELIS S; PROSEN DE; SCHAAD NW

来源：Gene, 1995, 166(1): 83-87.

DOI：10.1016/0378-1119(95)00569-5

• PHASEOLOTOXIN
• BEAN HALO BLIGHT
• ORNITHINE CARBAMOYLTRANSFERASE
• PSEUDOMONAS SYRINGAE

Part of an apparent open reading frame in the tox-argK gene cluster of Pseudomonas syringae pathovar phaseolicola (Psp) potentially encodes a polypeptide with sequence similarity to fatty acid desaturases (DES). Escherichia coli B expressing this segment under T7 promoter control produced a 34-kDa polypeptide. The possible involvement of a DES in facilitating phaseolotoxin (Ptx) secretion at the low temperatures normally required for its synthesis and the evolutionary implications about the origin of the tox-argK gene cluster are discussed.

• 出版日期1995-12-1