A monoclonal antibody recognizes undifferentiation-specific carbohydrate moieties expressed on cell surface of the human dental pulp cells

作者：Kang Kyung Jung; Ko Seon Yle; Ryu Chun Jeih; Jang Young Joo^*

来源：Stem Cell Research, 2017, 21: 85-93.

DOI：10.1016/j.scr.2017.04.002

• Human dental pulp cells (hDPCs)
• Undifferentiation-specific surface marker
• Glycosylation

Human dental pulp cells are obtained fromdental pulp tissue, and have the ability to formdentin and a pulp-like complex. Although adult stem cells have been identified from the primary culture by using specific cell surface markers, the identity of surface markers for the purification of stem cells within the dental pulp population are still unclear. Previously, we had constructed monoclonal antibodies against the undifferentiated cell-specific surface markers of human dental pulp cells (hDPCs) by performing decoy immunization. Among them, a monoclonal antibody against the cell surface antigen of the undifferentiated hDPCs (named UPSA-1) was purified and its heavy and light chain consensus regionswere analyzed. The cell surface binding affinity of UPSA-1mAb on the undifferentiated hDPCs was stronger than that on the differentiated cells. When tunicamycin was applied to hDPSCs during culture, the cell surface binding affinity of the antibody was dramatically decreased, and dentinogenic differentiation was reduced. The purified UPSA-1 antigen band resulting from immunoprecipitation disappeared or shifted down on the SDS-PAGE by deglycosylation. These data suggested that glycosylation on the cell surface might be a marker of an undifferentiated state, and that UPSA-1 mAb might be useful for identifying the carbohydrate moiety on the cell surface of undifferentiated pulp cells.

• 出版日期2017-5