Molecular Cloning, Expression and Characterization of a Squalene Synthase Gene from Grain Amaranth (Amaranthus cruentus L.)

作者：Park Young Jun; Nemoto Kazuhiro; Minami Mineo; Matsushima Kenichi; Nomura Tomohide; Kinoshita Jun ichi; Nishikawa Tomotaro^*

来源：Japan Agricultural Research Quarterly, 2016, 50(4): 307-317.

DOI：10.6090/jarq.50.307

• Gene expression
• molecular phylogeny

A gene encoding squalene synthase from grain amaranth was cloned and characterized. The full-length cDNA was 1805-bp long and contained a 1248-bp open reading frame encoding a protein of 416 amino acids with a molecular mass of 47.6 kDa. Southern blot analysis revealed that the A. cruentus genome contained a single copy of the gene. Comparison of the cDNA and genomic sequences indicated that the amaranth SQS gene had 12 introns and 13 exons. All 13 exons contributed to the coding sequence. The predicted amino acid sequence of the SQS cDNA shared high homology with those of SQSs from several other plants. It contained five conserved domains that are believed to represent crucial regions of the active site. We conducted qRT-PCR analyses to examine the expression pattern of the SQS gene in seeds at different developmental stages and in several tissues. The amaranth SQS gene was expressed late in seed development and played a role in the perisperm, and mainly expressed in stem and root tissues.

• 出版日期2016-10