Selective sample pretreatment by molecularly imprinted polymer for the determination of LSD in biological fluids

作者：Chapuis Hugon Florence^*; Cruz Vera Marta; Savane Ramatoulaye; Ali Wassim Hadj; Valcarcel Miguel; Deveaux Marc; Pichon Valerie

来源：Journal of Separation Science, 2009, 32(19): 3301-3309.

DOI：10.1002/jssc.200900247

• Drugs
• Hair
• LC-MS
• MIP
• SPE

For the first time, a molecularly imprinted polymer (MIP) was synthesized by a non-covalent imprinting approach for the selective extraction of an illicit drug, LSD, from hair and urine samples. For the synthesis of MIP, an analog of LSD, was taken as a dummy template, methacrylic acid as a functional monomer, and ACN as a porogen solvent. The MIP was used for offline extraction before HPLC-MS analysis. By studying the interactions taking place between the LSD and the MIP, a selective procedure was established in organic media and applied to hair samples. By this way, 0.1 ng/mg of LSD was successfully detected in hair with 82% of extraction recovery. A low retention was also obtained on the control polymer (only 9%). This procedure was then modified to obtain a selective extraction in aqueous media for the determination of LSD in urine samples. The comparison with a conventional C,8 clearly demonstrated the selectivity brought by the MIP to the determination of LSD in urine. LSD was easily detected in urine at only 0.5 ng/mL with 83% of extraction recovery on the MIP and 11% on the NIP. An LOQ of 0.2 pg/mL was estimated in urine samples.

• 出版日期2009-10