科研之友
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摘要
In auditory pathways, the precision of action potential (AP) propagation depends on axon myelination and high densities of voltage-gated Na (Na-v) channels clustered at nodes of Ranvier. Changes in Na-v channel expression at the heminode, the final node before the nerve terminal, can alter AP invasion into the presynaptic terminal. We studied the activity-dependent formation of Na-v channel clusters before and after hearing onset at postnatal day 12 in the rat and mouse auditory brain stem. In rats, the Na-v channel cluster at the heminode formed progressively during the second postnatal week, around hearing onset, whereas the Na-v channel cluster at the nodes was present before hearing onset. Initiation of heminodal Na-v channel clustering was correlated with the expression of scaffolding protein ankyrinG and paranodal protein Caspr. However, in whirler mice with congenital deafness, heminodal Na-v channels did not form clusters and maintained broad expression, but Na-v channel clustering was normal at the nodes. In addition, a clear difference in the distance from the heminodal Na-v channel to the calyx across the mediolateral axis of the medial nucleus of the trapezoid body (MNTB) developed after hearing onset. In the medial MNTB, where neurons respond best to high-frequency sounds, the heminodal Na-v channel cluster was located closer to the terminal than in the lateral MNTB, where neurons respond best to low-frequency sounds. Thus sound-mediated neuronal activities are potentially associated with the refinement of the heminode adjacent to the presynaptic terminal in the auditory brain stem.
- 出版日期2017-2
