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摘要
BackgroundIntradermal testing is useful for the identification of environmental allergens to which cats could be hypersensitive; intradermal test reactions are often subtle and difficult to interpret in cats. Percutaneous testing is the standard technique for the detection of significant environmental allergens in people, but it has not yet been evaluated in cats with hypersensitivity dermatitis. %26lt;br%26gt;Hypothesis/ObjectivesThe purpose of this study was to evaluate and compare the skin test responses of healthy cats to percutaneous application and intradermal injections of control solutions. %26lt;br%26gt;MethodsTen clinically healthy cats were studied. Percutaneous applications of 0.0275 and 0.1mg/mL aqueous histamine, 6mg/mL glycerinated histamine, 0.9% buffered saline and 50% glycerosaline solution were performed using Greer Pick (Greer Laboratories, Lenoir, NC, USA) and Duotip-Test II (Lincoln Diagnostics, Decatur, IL, USA) percutaneous applicators. Reactions were compared with intradermal injections of 0.0275mg/mL aqueous histamine and 0.9% buffered saline as controls. %26lt;br%26gt;ResultsPositive responses to histamine solutions were significantly greater with the Greer Pick than with the Duotip-Test II. There were no significant differences between the histamine reactions using the Greer Pick applicator and the intradermal injections. Percutaneous reactions to histamine were more well demarcated and easier to read than intradermal injection reactions. Reactions to the saline controls were not noted. %26lt;br%26gt;Conclusions and clinical importancePercutaneous application of 6mg/mL glycerinated histamine solution, 50% glycerosaline solution and 0.9% buffered saline produced similar positive and negative control wheals. These observations warrant further studies of percutaneous allergen testing in cats with hypersensitivity dermatitis. %26lt;br%26gt;Resume %26lt;br%26gt;ContexteLes tests intradermiques sont utiles pour l%26apos;identification des allergenes environnementaux auxquels les chats pourraient etre sensibilises; les intradermoreactions sont souvent subtiles et difficiles a interpreter chez le chat. Les tests percutanes sont la technique standard de detection des allergenes environnementaux significatifs chez l%26apos;homme mais n%26apos;ont pas encore ete evalues chez les chats atteints de dermatite par hypersensibilite. %26lt;br%26gt;Hypotheses/ObjectifsLe but de cette etude etait d%26apos;evaluer et de comparer les reponses aux tests cutanes de chats sains a l%26apos;application percutanee et aux injections intradermiques de solutions de controle. %26lt;br%26gt;MethodesDix chats cliniquement sains ont ete enroles. Les applications percutanees de 0.0275 et 0.1mg/mL d%26apos;histamine aqueuse, 6mg/mL d%26apos;histamine glycerinee, 0.9% de solution saline tampon et 50% de solution glycerosaline ont ete realisees avec des applicateurs percutanes Greer Pick (Greer Laboratories, Lenoir, NC, USA) et Duotip-Test II (Lincoln Diagnostics, Decatur, IL, USA). Les reactions ont ete comparees avec des injections intradermiques controles de 0.0275mg/mL d%26apos;histamine aqueuse et 0.9% de saline tampon. %26lt;br%26gt;ResultatsLes reponses positives aux solutions d%26apos;histamine etaient significativement plus importantes avec Greer Pick qu%26apos;avec le Duotip-Test II. Il n%26apos;y avait aucune difference significative entre les reactions a l%26apos;histamine utilisant l%26apos;applicateur Greer Pick et les injections intradermiques. Les reactions percutanees a l%26apos;histamine etaient mieux demarquees et plus faciles a lire que les reactions aux injections intradermiques. Aucune reaction aux solutions controles de saline n%26apos;a pas ete observee. %26lt;br%26gt;Conclusions et importance cliniqueLes applications percutanees de 6mg/mL de solution d%26apos;histamine glycerinee, 50% de solution glycerosaline et 0.9% de saline tampon produisent des reactions circulaires controles positives et negatives identiques. Ces observations necessitent davantage d%26apos;etudes des tests percutanes d%26apos;allergenes chez le chat atteint de dermatite par hypersensibilite.
- 出版日期2013-10
