Application of near-infrared reflectance spectroscopy to the evaluation of rutin and D-chiro-inositol contents in tartary buckwheat

作者：Yang Nan; Ren Guixing^*

来源：Journal of Agricultural and Food Chemistry, 2008, 56(3): 761-764.

DOI：10.1021/jf072453u

• rutin
• D-chiro-inositol
• NIRS
• tartary buckwheat

Tartary buckwheat [Fagopyrum tataricum (L.) Gaench] is rich in rutin and D-chiro-inositol (DCI), which have beneficial effects in the treatment of hemorrhagic diseases and insulin-resistant diseases, respectively. The current methods of extraction and detection of rutin and DCI are complex and time-consuming; a simple way of analyzing these compounds in the native matrix would be desirable. In this work, near-infrared reflectance spectroscopy (NIRS) was applied to determine the contents of rutin and DCI in tartary buckwheat. The spectral data were compared with those determined from high-performance liquid chromatography (HPLC) methods. Models for predicting rutin and DCI contents in buckwheat were developed using a partial least-squares algorithm. Cross-validation procedures indicated good correlations between HPLC data and NIRS predictions (R(2)=0.76 for rutin and R(2)=0.86 for DCI). The rutin content ranged from 0.998 to 1.751%, while the DCI content covered 0.179 - 0.200%. The results showed that NIRS, a well-established and widely applied technique, could be applied to determine rutin and DCI in tartary buckwheat rapidly and nondestructively.

• 出版日期2008-2-13
• 单位中国农业科学院