MicroRNA-100 inhibits osteosarcoma cell proliferation by targeting Cyr61

作者：Huang, Jianhua; Gao, Kanda; Lin, Jian; Wang, Qiugen^*

来源：Tumor Biology, 2014, 35(2): 1095-1100.

DOI：10.1007/s13277-013-1146-8

• Osteosarcoma
• MicroRNA
• Cell proliferation
• miR-100
• Cyr61

Increasing evidence indicates that microRNAs (miRNAs) participate in almost every step of cellular processes and are often aberrantly expressed in human cancer. Therefore, the discovery of miRNAs may provide a new and powerful tool for understanding the mechanism and treatment of carcinogenesis. The aim of this study was to investigate the functional significance of miR-100 and to identify its possible target genes in osteosarcoma (OS) cells. Here, we found that expression level of miR-100 was significantly decreased in osteosarcoma tissues in comparison with the adjacent normal tissues. The enforced expression of miR-100 was able to inhibit cell proliferation in Saos-2 and MG63 cells, while its antisense oligonucleotides (antisense miR-100) promoted cell proliferation. Moreover, our results further revealed that expression of Cyr61, an extracellular matrix-associated growth factor, was negatively regulated by miR-100. Therefore, we consider that miR-100 acts as a tumor suppressor for osteosarcoma. It may provide novel diagnostic and therapeutic options for human osteosarcoma in the future.

• 出版日期2014-2
• 单位上海交通大学