Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 mu Var infection

作者：Corporeau Charlotte^*; Tamayo David; Pernet Fabrice; Quere Claudie; Madec Stephanie

来源：Journal of Proteomics, 2014, 109: 176-187.

DOI：10.1016/j.jprot.2014.06.030

• Marine bivalves
• Crassostrea gigas
• Herpesvirus
• Metabolism
• Warburg

Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 mu Var) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters exhibited an increased glycolysis and VDAC accumulation, which reflect a %26quot;Warburg effect%26quot; as initially reported in cancer cells and more recently in shrimp infected with virus. The results presented here should be useful for identifying potential biomarkers of disease resistance and developing antiviral measures.

• 出版日期2014-9-23