Hsp90, Hsp60 and HSF-1 genes expression in muscle, heart and brain of thermally manipulated broiler chicken

作者：Al Zghoul Mohammad Borhan^*; Ismail Zuhair Bani; Dalab Abd Elhafeed S; Al Ramadan Abdulla; Althnaian Thnaian A; Al ramadan Saeed Y; Ali Abdelhadi M; Albokhadaim Ibrahim F; Al Busadah Khalid Ahmed; Eljarah Abdulhakeem; Jawasreh Khaleel I; Hannon Kevin M

来源：Research in Veterinary Science, 2015, 99: 105-111.

DOI：10.1016/j.rvsc.2014.12.014

• Heat shock protein 90 (Hsp90)
• Heat shock protein 60 (Hsp60)
• Heat shock factor-1 (HSF-1)
• Thermotolerance
• Thermal manipulation

The effect of thermal manipulation (TM) during embryogenesis (ED 12-18) on mRNA expressions of heat shock proteins (Hsp90, Hsp60 and HSF-1) in muscle, heart and brain tissues during thermal challenge (TC) at post-hatching days 10 and 28 was investigated. Fertile chicken eggs were randomly divided into four groups: Control group (37.8 degrees C), TM1 (39 degrees C for 9 h), TM2 (39 degrees C for 12 h) and TM3 (39 degrees C for 18 h). At days 10 and 28 of age, chicks in TC groups were subjected to thermal challenge (TC) at 43.0 degrees C for 6 h while naive chicks were kept under regular conditions. When compared with the control, TM resulted in a significant increase in mRNA levels of Hsp90, Hsp60 and HSF-lin muscle, heart and brain tissues during embryogenesis and during TC at days 10 and 28 post-hatching. These results indicate a long-term enhancement of Hsp90, Hsp60 and HSF-1 gene expressions associated with improved thermotolerance acquisition in thermally manipulated chicks.

• 出版日期2015-4