Analysis of S-allele genetic diversity in Sicilian almond germplasm comparing different molecular methods

作者：Curro, Sergio; La Malfa, Stefano; Distefano, Gaetano^*; Long, Guiyou; Sottile, Francesco; Gentile, Alessandra

来源：Plant Breeding, 2015, 134(6): 713-718.

DOI：10.1111/pbr.12318

• consensus and specific primers
• Prunus dulcis (Miller)
• self-(in)compatibility
• S-genotyping
• S-RNase
• sequencing

Italian almond germplasm is characterized by a wide diversity in several growing areas among which Sicily is one of the most important. Analysis with consensus and specific primers and DNA sequencing was performed to investigate S-RNase genetic diversity and to elucidate the homology rate within a genetic pool of 27 Italian accessions. Interestingly, some of the self-compatible cultivars did not show the presence of S-f allele. Amplicons from consensus and allele-specific PCR primers revealed a high level of variability. Sequencing of all the S-RNase amplicons derived from consensus primers allowed the identification of two new S-RNase alleles (S-51 and S-52). Surprisingly, despite the AA replacement mutation, S51 did not exhibit any change of its S-RNase function. Additionally, several mutations, with no effect on amino acid composition, were detected in the intron and/or in the ORF of four known alleles (S-g, S-10, S-31 and S-35). Genetic variation, regarding point mutations and only detected by sequencing, was revealed among 11 of 27 tested cultivars. The new sources of variability might have an interest for product traceability.

• 出版日期2015-12
• 单位湖南农业大学