Decolorization of sulfonated azo dyes with two photosynthetic bacterial strains and a genetically engineered Escherichia coli strain

作者：Liu Guang fei^*; Zhou Ji ti; Qu Yuan yuan; Ma Xin

来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2007, 23(7): 931-937.

DOI：10.1007/s11274-006-9316-0

• azo dye
• azoreductase
• decolorization
• photosynthetic bacteria
• Rhodobacter sphaeroides
• Rhodopseudomonas palustris

Sulfonated azo dyes were decolorized by two wild type photosynthetic bacterial (PSB) strains (Rhodobacter sphaeroides AS1.1737 and Rhodopseudomonas palustris AS1.2352) and a recombinant strain (Escherichia coli YB). The effects of environmental factors (dissolved oxygen, pH and temperature) on decolorization were investigated. All the strains could decolorize azo dye up to 900 mg l(-1), and the correlations between the specific decolorization rate and dye concentration could be described by Michaelis-Menten kinetics. Repeated batch operations were performed to study the persistence and stability of bacterial decolorization. Mixed azo dyes were also decolorized by the two PSB strains. Azoreductase was overexpressed in E. coli YB; however, the two PSB strains were better decolorizers for sulfonated azo dyes.

• 出版日期2007-7
• 单位大连理工大学