Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides

作者：Dubois Camille; Campbell Meghan A; Edwards Stacey L; Wengel Jesper; Veedu Rakesh N^*

来源：Chemical Communications, 2012, 48(44): 5503-5505.

DOI：10.1039/c2cc31316b

Enzymatic recognition of unlocked nucleic acid (UNA) nucleotides was successfully accomplished. Therminator DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of a 81 mer UNA-modified DNA library was efficiently achieved by KOD DNA polymerase.

• 出版日期2012