科研之友
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摘要
Aim: To explore the clinical significance of Neuropilin 2 (NRP2) and vascular endothelial growth factor-C (VEGF-C) in gastric adenocarcinoma. Method: We detected the expression of VEGF-C and NRP2 in 92 patients with gastric adenocarcinoma with immunohistochemistry (IHC) and analyzed the correlation between VEGF-C, NRP2 expression and clinicopathologic factors. With RT-PCR, we analyzed the mRNA level of VEGF-C and NRP2 in tumor tissue and corresponding tissue, tumor in situ and invaded lymph nodes. With transwell assay, we estimated the role of VEGF-C and NRP2 in cell invasion of gastric adenocarcinoma. Results: The high-expression rates of VEGFC, NRP2 and VEGF-C&NRP2 were 52.17%, 33.70% and 13.04%, respectively. VEGF-C&NRP2 high expression was significantly associated with positive lymph node metastasis (P= 0.009). The mRNA level of NRP2 in tumor tissue was significantly higher than that in adjacent tumor tissue (P= 0.030), and mRNA levels of both NRP2 and VEGF-C in invaded lymph nodeswereremarkably higher than those in the gastric adenocarcinoma in situ (P= 0.020 and 0.010, respectively). Human recombinant VEGF-C at 10 ng/ml could promote invasion of gastric adenocarcinoma cells, and this effect was significantly reduced after NRP2 knockdown, indicating NRP2 was required in VEGF-C induced gastric adenocarcinoma invasion. Conclusion: High expression of VEGF-C&NRP2 was significantly associated with positive lymphatic invasion of gastric adenocarcinoma. VEGF-C could promote the gastric adenocarcinoma cell invasion via stimulating NRP2, indicating that VEGF-C-NRP2 signaling pathway could be a potential blocking target to reduce the lymphatic invasion.
