Ageing and colony-forming efficiency of human hair follicle keratinocytes

作者：Lecardonnel Jennifer; Deshayes Nathalie; Genty Gaianne; Parent Nathalie; Bernard Bruno A; Rathman Josserand Michelle; Paris Maryline^*

来源：Experimental Dermatology, 2013, 22(9): 604-606.

DOI：10.1111/exd.12204

• ageing
• clone-forming efficiency
• holoclones
• human hair follicle keratinocytes

The decline of tissue regenerative potential of skin and hair is a hallmark of physiological ageing and may be associated with age-related changes in tissue-specific stem cells and/or their environment. Human hair follicles (hHF) contain keratinocytes having the property of stem cells such as clonogenic potential. Growth capacity of hHF keratinocytes shows that most of the colony-forming cells are classified as holoclones, meroclones or paraclones when analysed in a clonal assay (Cell, Volume 76, page 1063). Despite the well-known impact of ageing on human hair growth, little is known about changes in hHF keratinocyte clonogenic potential with age. This study aimed at assessing the clone-forming efficiency (CFE) of hHF keratinocytes from three age groups of human donors. It demonstrates that ageing affects hHF keratinocyte CFE.

• 出版日期2013-9