Tumor necrosis factor-alpha (TNF-alpha)-mediated in vitro human retinal pigment epithelial (RPE) cell migration mainly requires Akt/mTOR complex 1 (mTORC1), but not mTOR complex 2 (mTORC2) signaling

作者：Liu Yuan; Cao Guo Fan; Xue Jinsong; Wan Jerry; Wan Yinsheng; Jiang Qing; Yao Jin^*

来源：European Journal of Cell Biology, 2012, 91(9): 728-737.

DOI：10.1016/j.ejcb.2012.03.008

• Proliferative vitreoretinopathy (PVR)
• TNF-alpha
• RPE cell migration
• Akt/mTOR signaling

When rhegmatogenous retinal detachment occurs, tumor necrosis factor-alpha (TNF-alpha) among other cytokines leaks into the subretinal space, induces resident retinal pigment epithelial (RPE) cells to migrate, which is the initial step of proliferative vitreoretinopathy (PVR). In the current study, we aim to understand how this is regulated by focusing the cellular mechanisms involved. Here we indentified an Akt/Tuberous sclerosis protein 2 (TSC2)/mTOR complex1 (mTORC1) signaling pathway after TNF-alpha treatment to mediate RPE cell migration. Suppression of mTORC1 activation, either by its inhibitor rapamycin, or by activation of its suppressor AMP activated protein kinase (AMPK), inhibited TNF-alpha mediated RPE cell migration, while RNA interference (RNAi)-mediated knocking-down of SIN1 or Rictor, two key components of mTOR complex 2 (mTORC2), had no significant effect on TNF-alpha-induced RPE cell migration. Our data provide initial evidence that TNF-alpha-mediated in vitro RPE cell migration mainly requires Akt/mTORC1, but not mTORC2 signaling. The results of this study may lead to indentify novel signaling targets against PVR.

• 出版日期2012-9
• 单位南京医科大学