A simple and selective method for the measurement of leucine and isoleucine from plasma using electrospray ionization tandem mass spectrometry

作者：Bishop Michael J^*; Crow Brian; Norton Dean; Kovalcik Kasey; George Joe; Bralley J Alexander

来源：Rapid Communications in Mass Spectrometry, 2007, 21(12): 1920-1924.

DOI：10.1002/rcm.3044

An analytical method was developed for the rapid and accurate quantification of leucine (LEU) and isoleucine (ILE) from plasma using electrospray ionization tandem mass spectrometry (ESI-MS/MS). The two isomeric amino acids were selectively detected using fragment ions unique to each compound. As a result, the need for chromatographic separation was avoided allowing for faster analysis (3 min). The possibility of any considerable interference between the two transitions was closely monitored, with no significant interference being observed. The presence of interfering compounds in plasma was also evaluated and found to minimal. The method was evaluated based upon linearity, with r(2) >= 0.995 for both compounds, and accuracy, with no more than 8% deviation from the theoretical value.

• 出版日期2007