NRAMP1 and hGPX1 Gene Polymorphism and Response to Bacillus Calmette-Guerin Therapy for Bladder Cancer

作者：Chiong Edmund^*; Kesavan Arshvin; Mahendran Ratha; Chan Yiong Huak; Sng Jen Hwei; Lim Yew Koon; Kamaraj Revathi; Tan Theresa M C; Esuvaranathan Kesavan

来源：European Urology, 2011, 59(3): 430-437.

DOI：10.1016/j.eururo.2010.11.031

• Bacillus Calmette-Guerin
• Bladder cancer
• Gene polymorphism

Background: The natural resistance-associated macrophage protein 1 (NRAMP1) gene is associated with susceptibility to Mycobacterium tuberculosis in humans and to bacillus Calmette-Guerin (BCG) in mice. The detoxification enzyme, human glutathione peroxidase 1 (hGPX1), is associated with recurrence of bladder cancer (BCa).
Objective: To determine whether NRAMP1 and hGPX1 gene polymorphisms correlate with response to BCG immunotherapy for non-muscle-invasive BCa (NMIBC).
Design, setting, and participants: DNA was obtained from the peripheral blood of 99 NMIBC patients who were prospectively randomized to receive postresection intravesical BCG (81 mg [n = 50] or 27 mg [n = 19]) or BCG (27 mg) with interferon alpha (IFN-alpha; n = 30). The median follow-up time was 60 mo.
Intervention: Intravesical BCG or BCG-IFN-alpha.
Measurements: Restriction fragment length polymorphism (RFLP) analysis was performed to identify polymorphisms in the NRAMP1 promoter region (GT repeat number) and at position 543 (aspartate [D] and/or asparagine [N] expression) within the NRAMP1 protein (D543N) and position 198 (proline and/or leucine expression) within the hGPX1 protein (Pro198Leu). Data were analyzed using chi(2) analysis, multivariate analysis, and Kaplan-Meier curves.
Results and limitations: On univariate analysis, the NRAMP1 D543N G: G genotype had decreased cancer-specific survival (CSS; p = 0.036). The hGPX1 CT genotype (Pro-Leu) had decreased recurrence time (p = 0.03) after BCG therapy. On multivariate analysis, patients with the NRAMP1 D543N G: G genotype and allele 3 (GT) n polymorphism had decreased recurrence time (p = 0.014 and p = 0.03) after BCG therapy. The limitation of this study was its small sample size.
Conclusions: Polymorphisms of the NRAMP1 and hGPX1 genes may be associated with recurrence of BCa after BCG immunotherapy.

• 出版日期2011-3