科研之友
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摘要
Objective: Warthin%26apos;s tumors and oncocytomas show exceptionally good (TcO4-)-Tc-99m (Tc) accumulation images in Tc scintigraphy. However, the mechanism of Tc accumulation in these tumors remains unclear. Sodium-iodide symporter (NIS) is a plasma membrane protein expressed in the thyroid, lactating breast, stomach and salivary glands; it facilitates uptake of I and Tc. We hypothesized that Warthin%26apos;s tumor cells and oncocytomas may also express NIS, which would promote uptake of Tc. We examined NIS localization and the mechanism of Tc accumulation in various salivary gland tissues. %26lt;br%26gt;Methods: Immunohistological localization of NIS was performed for 19 tumors from 18 patients who underwent preoperative Tc scintigraphy. Expression of mRNA for NIS in the normal salivary gland, Warthin%26apos;s tumors and pleomorphic adenomas was analyzed by real-time PCR. %26lt;br%26gt;Results: In normal salivary glands, striated duct cells were strongly immunostained by anti-NIS antibodies. In Warthin%26apos;s tumors, eosinophilic epithelial cells exhibited positive immunostaining, but their staining was varied among the cases. Furthermore, all Tc-positive specimens were NIS-positive, and all Tc-negative specimens were NIS-negative. Real-time PCR showed that NIS mRNA expression was detectable in normal salivary glands and Warthin%26apos;s tumor cells. The expression was significantly higher in normal salivary glands compared with Warthin%26apos;s tumor cells and pleomorphic adenoma. %26lt;br%26gt;Conclusion: IC-positive salivary glands expressed NIS. Our findings suggest that Tc accumulation in Warthin%26apos;s tumors and oncocytomas is due to poorer Tc excretory function compared with normal salivary gland tissues, in addition to active uptake of (TcO4-)-Tc-99m via NIS.
- 出版日期2014-12
