Differentiation and characterization of embryonic stem cells into three germ layers

作者：Shiraki Nobuaki; Higuchi Yuichiro; Harada Seiko; Umeda Kahoko; Isagawa Takayuki; Aburatani Hiroyuki; Kume Kazuhiko; Kume Shoen^*

来源：Biochemical and Biophysical Research Communications, 2009, 381(4): 694-699.

DOI：10.1016/j.bbrc.2009.02.120

• ES cells
• Neuroectoderm
• Mesoderm
• Endoderm
• Pancreatic differentiation
• In vitro differentiation
• Growth factors

Embryonic stem cells differentiated on M15 cells have previously been shown to give rise to cells of the mesendodermal and definitive endodermal lineages. Here we demonstrate that neuroectodermal and mesodermal lineages can be derived from ES cells cultured on M15 cells and subsequently subjected to specific culture conditions, as confirmed by the expression of molecular markers. Prospective isolation and microarray analyses showed that neuroectodermal cells expressed anterior-to-posterior, as well as dorso-ventral regional markers, suggesting that this procedure could be used for the induction of cells belonging to a wide variety of neural lineages. Lateral mesoderm and paraxial mesoderm cells were also produced and their gene expression profiles were confirmed by microarray analyses. These results indicate that the M15 cell system provides a valuable tool for generating ES cell-derived lineage-specific cell types belonging to the three germ layers, namely neuroectoderm, mesoderm, and definitive endoderm.

• 出版日期2009-4-17