Differential Effects of Low- and High-dose Glucocorticoids on the Innate Immunity of Corneal Epithelium in Vitro

作者：Xie, Xin; Yan, Xiaoran; Lin, Zhenyun; Jin, Xiuming^*

来源：Ocular Immunology and Inflammation, 2011, 19(4): 275-281.

DOI：10.3109/09273948.2011.569110

• TOLL-LIKE RECEPTORS
• STAPHYLOCOCCUS-AUREUS INFECTION
• ANTIINFLAMMATORY ACTION
• INDUCED EXPRESSION
• ADAPTIVE IMMUNITY
• GENE-EXPRESSION
• MAP KINASE
• CELLS
• MECHANISMS
• RESPONSES

Methods: Human corneal epithelial cell lines (HCEC) were co-cultured with LPS and hydrocortisone to determine whether hydrocortisone modulates the expression and function of TLR2, 4. The release of IL-6, 8 from cultured HCEC was measured in the presence and absence of specific blocking antibodies to TLR2, 4. The proteins of TLR2, 4 were also compared by Western blot.
Results: Incubation of HCEC with LPS upregulated the expression of TLR2, 4 and increased the release of IL-6, 8. This upregulation was enhanced by low-concentration hydrocortisone, but inhibited by high-concentration hydrocortisone. The concentration of IL-6, 8 was also enhanced by low-concentration hydrocortisone and inhibited by high-concentration hydrocortisone.
Conclusions: Low-concentration hydrocortisone enhances the expression and function of TLRs in HCEC and provides evidence for a novel function of glucocorticoids in innate immunity.

• 出版日期2011-8
• 单位杭州市第一人民医院; 青岛市市立医院; 浙江大学