Development of a dual nanocarrier system as a potential stratagem against amyloid- induced toxicity

作者：Kumaraswamy Priyadharshini; Sethuraman Swaminathan; Krishnan Uma Maheswari^*

来源：Expert Opinion on Drug Delivery, 2014, 11(8): 1131-1147.

DOI：10.1517/17425247.2014.912211

• Alzheimer's disease
• amyloid beta
• curcumin
• liposomes
• Tjernberg peptide

Objective: Therapeutic formulation to reduce amyloid beta (A beta) insult in neuronal cells remains an important focus in the treatment of Alzheimer's disease. To combat the multifactorial threats that arise during amyloid plaque formation, multi-dimensional approach is required.
Methods: Peptide sequence KLVFF derived from the core recognition motif of A beta(1) (-) (42) can bind to the plaques and help to reduce further accumulation. In our previous work, we have reported various self-assembling structures of KLVFF along with its surface tension lowering ability to overcome the cytotoxicity caused by A beta(1) (-) (42). In the present work, we have developed a novel combination of peptide-curcumin-loaded liposomal formulation and characterized for its morphology, protein adsorption and colloidal stability. The therapeutic efficacy of the formulation was tested using a cholinergic neuronal cell line pre-treated with A beta(1 - 42).
Results: The physiochemical characterization and in vitro efficacy of peptide- curcumin-loaded liposomal formulation were found to outperform well in bringing down the amyloid toxicity.
Conclusion: This cumulative evidence indicates that the nanocarrier-based alternative treatment stratagem is an effective way to treat Alzheimer's disease.

• 出版日期2014-8