Multicentric occurrence of multiple papillary thyroid carcinomas -HUMARA and BRAF mutation analysis

作者：Nakazawa Tadao^*; Kondo Tetsuo; Tahara Ippei; Kasai Kazunari; Inoue Tomohiro; Oishi Naoki; Mochizuki Kunio; Kubota Takeo; Katoh Ryohei

来源：Cancer Medicine, 2015, 4(8): 1272-1280.

DOI：10.1002/cam4.466

• BRAF mutation
• clonal analysis
• HUMARA
• intraglandular metastasis
• multicentric occurrence
• multiple papillary thyroid carcinoma
• X-chromosome inactivation pattern

Papillary thyroid carcinomas (PTCs) occasionally form multiple tumor foci in different sites of the same thyroid gland. However, it is controversial whether discrete nodules of PTC arise independently (multicentric occurrence) or are seeded from a single tumor via lymphatic channels (intraglandular metastasis). In order to determine the clonal origin of multiple PTCs, we examined X-chromosome inactivation patterns using a human androgen receptor gene-based assay (HUMARA) and the BRAF mutation using allele-specific PCR (AS-PCR) in 32 microdissected cancerous tissues from 14 Japanese women with multifocal PTC. All tumor foci were greater than 3 mm in size and met the criteria for microscopic classical PTC. Samples from 13 of the 14 patients were informative based on HUMARA. Tumor foci from two cases (15.4%) displayed a discordant X-chromosome inactivation pattern. Foci from the other 11 cases (84.6%) showed a concordant inactivation pattern of the X-chromosome. AS-PCR indicated that BRAF mutational status between the tumor foci was discordant in three (25%) and concordant in nine (75%) of 12 available cases. When the results of these two molecular analyses were combined, 28.6% of the cases were discordant in X-chromosome inactivation pattern and/or BRAF mutation, suggesting multicentric origin. Some of the remaining concordant cases also may be of multicentric origin. These results support a hypothesis that multicentric occurrence in multiple PTCs may be common, possibly greater than 30%. Although the exact mechanism of multicentric occurrence is still unclear, our findings contribute to the understanding the histogenesis of papillary thyroid carcinoma.

• 出版日期2015-8