A regulatory toolbox of MiniPromoters to drive selective expression in the brain

作者：Portales Casamar Elodie; Swanson Douglas J; Liu Li; de Leeuw Charles N; Banks Kathleen G; Sui Shannan J Ho; Fulton Debra L; Ali Johar; Amirabbasi Mahsa; Arenillas David J; Babyak Nazar; Black Sonia F; Bonaguro Russell J; Brauer Erich; Candido Tara R; Castellarin Mauro; Chen Jing; Chen Ying; Cheng Jason C Y; Chopra Vik; Docking T Roderick; Dreolini Lisa; D'Souza Cletus A; Flynn Erin K; Glenn Randy; Hatakka Kristi; Hearty Taryn G; Imanian Behzad

来源：Proceedings of the National Academy of Sciences, 2010, 107(38): 16589-16594.

DOI：10.1073/pnas.1009158107

• knockin mice
• neuroscience
• promoter design
• selective expression
• transcription

The Pleiades Promoter Project integrates genomewide bioinformatics with large-scale knockin mouse production and histological examination of expression patterns to develop MiniPromoters and related tools designed to study and treat the brain by directed gene expression. Genes with brain expression patterns of interest are subjected to bioinformatic analysis to delineate candidate regulatory regions, which are then incorporated into a panel of compact human MiniPromoters to drive expression to brain regions and cell types of interest. Using single-copy, homologous-recombination "knockins" in embryonic stem cells, each MiniPromoter reporter is integrated immediately 5 ' of the Hprt locus in the mouse genome. MiniPromoter expression profiles are characterized in differentiation assays of the transgenic cells or in mouse brains following transgenic mouse production. Histological examination of adult brains, eyes, and spinal cords for reporter gene activity is coupled to costaining with cell-type-specific markers to define expression. The publicly available Pleiades MiniPromoter Project is a key resource to facilitate research on brain development and therapies.

• 出版日期2010-9-21