A dual-signal colorimetric and near-infrared fluorescence probe for the detection of exogenous and endogenous hydrogen peroxide in living cells

作者：Zhou, Zile; Li, Yaqian; Su, Wei; Gu, Biao; Xu, Hai; Wu, Cuiyan; Yin, Peng; Li, Haitao^*; Zhang, Youyu

来源：Sensors and Actuators B: Chemical , 2019, 280: 120-128.

DOI：10.1016/j.snb.2018.09.126

• Hydrogen peroxide
• Near-infrared
• Dual signals
• Cells imaging

As a classic kind of reactive oxygen species (ROS), hydrogen peroxide (H2O2) plays a critical role in physiological and pathological processes. It is of significant importance to detect H2O2 with sufficient sensitivity, selectivity and universality in biological field. Herein a novel near-infrared probe (DCM-B) containing dicyanomethylene-4H-pyran as fluorophore and boronate ester group as the recognition unit was developed for the detection of H2O2. Experiments showed that the remarkable fluorescence enhancement and noticeable color change from yellow to purple were observed while H2O2 was added to DCM-B solution. It is proved that the boronate ester group of DCM-B cleavage and fluorescence off-on response based on intramolecular charge transfer (ICT) mechanism were obtained when the probe reacted with H2O2, and DCM-B showed excellent sensitivity, high selectivity to H2O2 detection in the presence of many competing analytes. Encouraged by the optical properties of near-infrared emission, it was successfully applied to the imaging of exogenous and endogenous hydrogen peroxide in living cells. More importantly, considering that the final metabolite of choline and glucose is H2O2 in living cells and organism, we expanded DCM-B to the indirect detection of choline and glucose with satisfactory performance.

• 出版日期2019-2-1
• 单位湖南师范大学