Dietary Supplementation with Omega-3 Polyunsaturated Fatty Acids Modulate Matrix Metalloproteinase Immunoreactivity in a Mouse Model of Pre-abdominal Aortic Aneurysm

作者：Kavazos Kristyn; Nataatmadja Maria; Wales Kathryn M; Hartland Elke; Williams Chloe; Russell Fraser D^*

来源：Heart Lung and Circulation, 2015, 24(4): 377-385.

DOI：10.1016/j.hlc.2014.11.005

• Abdominal aortic aneurysm
• Matrix metalloproteinases
• Long chain omega-3 polyunsaturated fatty acids
• Transforming growth factor-beta 1
• Cytokines
• Elastin

Background Two-day infusion of angiotensin II to apolipoprotein E-deficient (ApoE(-/-)) mice provides a model of preabdominal aortic aneurysm. Long chain omega-3 polyunsaturated fatty acids (n-3 PUFAs) have anti-inflammatory effects. This study examined the effect of an eight-week low or high n-3 PUFA diet in ApoE(-/-) mice on matrix metalloproteinase (MMP) expression and elastin degradation.
Methods ApoE(-/-) mice were fed a low or high n-3 PUFA diet for eight weeks prior to two-day infusion with angiotensin II. The omega-3 index, MMP-2, MMP-9, TIMP-1, and TGF-beta 1 immunoreactivity, and elastin fragmentation were measured.
Results The omega-3 index with the low and high n-3 PUFA diet was 3.78% and 13.03%, respectively. MMP-9 immunoreactive stain intensity was lower in mice fed the high, compared to the low n-3 PUFA diet in endothelial cells (suprarenal aorta), and inflammatory cells (suprarenal and infrarenal aorta). Inflammatory cells had higher TIMP-1 and TGF-beta 1 stain intensity in mice fed the high, compared to the low n-3 PUFA diet (suprarenal aorta). MMP-2 immunoreactivity was unaffected by diet. A non-significant trend for reduced elastin fragmentation was observed in mice fed the high n-3 PUFA diet.
Conclusion Dietary supplementation with n-3 PUFAs may have protective anti-inflammatory effects mediated through modulation of MMPs and TIMPs.

• 出版日期2015-4