A comparison of methods for purification and concentration of norovirus GII-4 capsid virus-like particles

作者：Huhti L^*; Blazevic V; Nurminen K; Koho T; Hytonen V P; Vesikari T

来源：Archives of Virology, 2010, 155(11): 1855-1858.

DOI：10.1007/s00705-010-0768-z

Noroviruses (NoVs) are one of the leading causes of acute gastroenteritis worldwide. NoV GII-4 VP1 protein was expressed in a recombinant baculovirus system using Sf9 insect cells. Several methods for purification and concentration of virus-like particles (VLPs) were evaluated. Electron microscopy (EM) and histo-blood group antigen (HBGA) binding assays showed that repeated sucrose gradient purification followed by ultrafiltration resulted in intact VLPs with excellent binding to H type 3 antigens. VLPs were stable for at least 12 months at 4 degrees C, and up to 7 days at ambient temperature. These findings indicate that this method yielded stable and high-quality VLPs.

• 出版日期2010-11
• 单位河北医科大学