Analyses of inheritance patterns and consistent expression of sporamin and chitinase PjChi-1 genes in Brassica napus

作者：Liu, HongBo; Naeem, Muhammad S.; Liu, Dan; Zhu, YaNa; Guo, Xiang; Cui, Peng; Zhou, WeiJun^*

来源：Plant Breeding, 2011, 130(3): 345-351.

DOI：10.1111/j.1439-0523.2010.01827.x

• Brassica napus
• progeny
• sporamin
• chitinase PjChi-1
• insect resistance
• antifungal

P>Stable and consistent expression of foreign genes in serial propagation is a crucial factor to induce effective and efficient resistance through genetic engineering in insects and fungi. To better understand the inheritance patterns and expression of transgenes, two genes (sporamin and chitinase PjChi-1) in T-1 progeny of four single-copy T-0 transformants were characterized. PCR detection revealed that the segregation ratio was 3 : 1 for tested plants of each T-0 transformant, which agreed with the Mendelian rules. In RT-PCR analysis, 10 positive progeny plants of T-0-11 line showed a stable expression of sporamin and PjChi-1 but not in wild type. Moreover, a higher expression level of sporamin gene in T-1-11-3 was confirmed compared to the other progeny plants using Northern blot hybridization technique. Artificial inoculation of 10 plants (T-1-11) with Lepidoptera larvae (Plutella xylostella) and fungus (Sclerotinia sclerotiorum) in in vitro bioassays showed significant resistance compared to the wild type. Progeny T-1-11-3 showed the stronger resistance, which was consistent with the higher expression level of sporamin gene in Northern blot hybridization detection, whereas there was no difference in insect and fungi resistance among these positive T-1-11 plants. These results indicate that both combined genes have been inherited from parental transformants to their progeny and showed a consistent resistance to insect and fungus in all positive T-1 progeny.

• 出版日期2011-6
• 单位浙江大学