Application of a Disposable Screen-Printed Electrode to Depression Diagnosis for Laboratory Rats Based on Blood Serotonin Detection

作者：Liu, Min; Hu, Jiajie; Ma, Tian; Wang, Shi; Ding, Hong^*

来源：Analytical Sciences, 2011, 27(8): 839-843.

DOI：10.2116/analsci.27.839

• GLASSY-CARBON ELECTRODE
• MILD STRESS
• MAJOR DEPRESSION
• RAPID DETECTION
• ANTIDEPRESSANT
• MEMBRANE
• DOPAMINE
• OXIDE
• NOREPINEPHRINE
• SYMPTOMS

No exact and digital diagnostic methods for depression have been found. In this study, we prepared a 5-HT biosensor based on screen-printed electrode and applied it to a rat depression model caused by chronic unpredictable mild stress (CUMS). During CUMS, the blood 5-HT and the depression behavior of the depressed rats and the rats treated by antidepressants were recorded. The correlation coefficient of 5-HT was 0.9966 (0 - 4 x 10(-6) M) on the sensor. Results demonstrated that 5-HT level of the depressed rats declined while the depression behavior was aggravated. Fluoxetine (20 mg/kg) and lentinan (10, 20 mg/kg) mildly elevated 5-HT level and slowly regulated the behavior. Mifepristone (20 mg/kg) and Rhizoma Coptidis water extract (10, 20, 100 mg/kg) quickly reversed 5-HT level and the depression behavior. This sensor can accurately test blood 5-HT and might be applied to rapid diagnosis for depression and evaluation of antidepressants effect.

• 出版日期2011-8
• 单位中南民族大学; 武汉大学