ASSESSMENT OF INTENSIVE CARE UNIT-ACQUIRED WEAKNESS IN YOUNG AND OLD MICE: AN E. coli SEPTIC PERITONITIS MODEL

作者：Witteveen Esther^*; Hoogland Inge C M; Wieske Luuk; Weber Nina C; Verhamme Camiel; Schultz Marcus J; Van Schaik Ivo N; Horn Janneke

来源：Muscle & Nerve, 2016, 53(1): 127-133.

DOI：10.1002/mus.24711

• animal model
• critical illness myopathy
• critical illness polyneuropathy
• grip strength
• intensive care unit-acquired weakness

Introduction: There are few reports of in vivo muscle strength measurements in animal models of ICU-acquired weakness (ICU-AW). In this study we investigated whether the Escherichia coli (E. coli) septic peritonitis mouse model may serve as an ICU-AW model using in vivo strength measurements and myosin/actin assays, and whether development of ICU-AW is age-dependent in this model. Methods: Young and old mice were injected intraperitoneally with E. coli and treated with ceftriaxone. Forelimb grip strength was measured at multiple time points, and the myosin/actin ratio in muscle was determined. Results: E. coli administration was not associated with grip strength decrease, neither in young nor in old mice. In old mice, the myosin/actin ratio was lower in E. coli mice at t = 48 h and higher at t = 72 h compared with controls. Conclusions: This E. coli septic peritonitis mouse model did not induce decreased grip strength. In its current form, it seems unsuitable as a model for ICU-AW.

• 出版日期2016-1