A new, simple, green, and one-pot four-component synthesis of bare and poly(alpha,gamma,l-glutamic acid)-capped silver nanoparticles

作者：Stevanovic Magdalena^*; Savanovic Igor; Uskokovic Vuk; Skapin Sreco D; Bracko Ines; Jovanovic Uros; Uskokovic Dragan

来源：Colloid and Polymer Science, 2012, 290(3): 221-231.

DOI：10.1007/s00396-011-2540-7

• Silver nanoparticles
• Green method
• Poly(alpha,gamma,L-glutamic acid)
• Capping agent
• In vitro toxicity

A simple and green chemical method has been developed to synthesize stable bare and capped silver nanoparticles based on the reduction of silver ions by glucose and capping by poly(alpha,gamma,l-glutamic acid) (PGA). The use of ammonia during synthesis was avoided. PGA has had a dual role in the synthesis and was used as a capping agent to make the silver nanoparticle more biocompatible and to protect the nanoparticles from agglomerating in the liquid medium. The synthesized PGA-capped silver nanoparticles in the size range 5-45 nm were stable over long periods of time, without signs of precipitation. Morphological examination has shown that the silver nanoparticles had a nearly spherical, multiply twinned structure. The effects of the reaction temperature and the reaction time during the synthesis were investigated too. The biocompatibility of the PGA-capped silver nanoparticles is discussed in terms of in vitro toxicity with human intestinal Caco-2 cells. The samples were characterized by UV-Visible spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and zeta potential measurements.

• 出版日期2012-2