科研之友
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摘要
The development of electronic sensors with minimized usage of reagents and washing steps in the sensing protocols will significantly facilitate the detection of biomolecules. In this work, by using a new pseudoknot,design of,the aptamer probes, the construction of an electronic sensor for reagentless and single-Step detection of immunoglobulin E (IgE) in human serum is described. The pseudoknot aptamer-probes-are self-assembled On the disposable electrode ,surface. The association of IgE, with the aptamer probes leads to conformational changes of the pseudoknot aptamer structures and brings, the redox-tags in close proximity to the electrode, resulting in amplified Current response for monitoring IgE. The effects: of the pseudoknot structure and the immobilitation concentration of the aptamer probes on the sensor performance are evaluated. Under optimal conditions,;the detection limit for IgE is estimated to be 60 pm. The sensor is also selective:land can be employed to detect IgE in human serum samples. The developed,sensor achieve reagentless, washing free and low-cost (with the disposable,electrode) electrochemical,detection of proteins, making this device a convenient sensing :platform for the monitoring of different biomarkers When coupled with the appropriate aptamer, probes.
