Diversification of Pseudomonas corrugata 2140 produces new phenotypes altered in GC-FAME, BIOLOG, and in vitro inhibition profiles and taxonomic identification

作者：Barnett SJ^*; Alami Y; Singleton I; Ryder MH

来源：Canadian Journal of Microbiology, 1999, 45(4): 287-298.

DOI：10.1139/cjm-45-4-287

• Pseudomonas corrugata
• variants
• phenotype plasticity
• FAME
• BIOLOG

Bacteria are known to rapidly produce new phenotypes, but it is unclear how phenotype "plasticity" relates to studies on the population ecology of bacteria in complex environments. We characterised a collection of 14 spontaneous phenotype variants, derived from in vitro and in vivo cultures (wheat roots) of Pseudomonas corrugata 2140, using fatty acid methyl ester profiles (GC-FAME), carbon substrate utilisation (BIOLOG), and in vitro inhibition against seven soil microorganisms. All three phenotype profiles indicated marked differences between some variants and the parent isolate. Some variant types were classified taxonomically by GC-FAME as different species to their wild-type parent, and up to a Euclidian distance of 11 from their parent. Taxonomic identification by the BIOLOG assay was more consistent; however, use of 22 carbon sources were altered (lost or gained) in one or more variants. All variant types had a reduced ability to inhibit one or more test organisms, depending on the variant and test organism. Hierarchical cluster analysis of variants using GC-FAME, BIOLOG, and inhibition profiles produced different groupings. The ability of variants to cross taxonomic boundaries specified by the GC-FAME and BIOLOG libraries at the species level has implications for both taxonomy and the ecological study of bacterial communities.

• 出版日期1999-4
• 单位CSIRO