Anti-Candida Activity of a Chitosan Hydrogel: Mechanism of Action and Cytotoxicity Profile

作者：Palmeira de Oliveira A^*; Ribeiro M P; Palmeira de Oliveira R; Gaspar C; Costa de Oliveira S; Correia I J; Pina Vaz C; Martinez de Oliveira J; Queiroz J A; Rodrigues A G

来源：Gynecologic and Obstetric Investigation, 2010, 70(4): 322-327.

DOI：10.1159/000314023

• Chitosan hydrogel
• Anticandida activity
• Biodegradable

Candida spp are common causative agents of mucocutaneous infections New therapeutic antifungal drugs are needed to treat chronic disease as these are frequently clinically resistant to azols Chitosan, among other possible vehicles for active compounds, shows an added value as it appears to have intrinsic antimicrobial properties The aim of the present study was to evaluate the anti-Candida activity of a medium-molecular-weight chitosan hydrogel (CH), to clarify its possible mechanism of action and to evaluate its cytotoxicity on human fibroblasts CH antifungal activity was assessed according to CLSI reference M27-A3 protocol, its mechanism of action was investigated by flow cytometry, and its cytotoxicity was studied by MTT assay CH demonstrated a full inhibition of C tropical's, C kruser, C guilliermondir and C parapsilosis growth while impairing C albicans and C glabrata viability Flow cytometry tests showed that CH acts by inducing primary lesion of the cytoplasmic membrane However, CH showed no cytotoxic effect upon human fibroblasts cells Resistant strains will require new therapeutic approa

• 出版日期2010