Analysis of testosterone in human urine using molecularly imprinted solid-phase extraction and corona discharge ion mobility spectrometry

作者：Mirmandieh Shiva; Mardihallaj Azam; Hashemian Zahra; Razavizadeh Jalal; Ghaziaskar Hassan; Khayamian Taghi^*

来源：Journal of Separation Science, 2011, 34(1): 107-112.

DOI：10.1002/jssc.201000583

• Ion mobility spectrometry
• Molecular imprinted polymer
• Testosterone

Analysis of testosterone was accomplished using corona discharge ion mobility spectrometry. Molecular imprinted polymer was used for the extraction and pre-concentration of testosterone. Analytical parameters including precision, dynamic range and detection limit were obtained. The linear dynamic range was from 10 to 250 ng/mL and the limit of detection was 0.9 ng/mL. The proposed method was used for analysis of testosterone in urine samples. A urine sample from a 3-year-old girl was used as the blank. The RSD was below 10%. The obtained results from the method were also compared with the standard method for analysis of testosterone using SPE-HPLC analysis. The results demonstrate the accuracy of the method.

• 出版日期2011-1