MTCH2/MIMP is a major facilitator of tBID recruitment to mitochondria

作者：Zaltsman Yehudit; Shachnai Liat; Yivgi Ohana Natalie; Schwarz Michal; Maryanovich Maria; Houtkooper Riekelt H; Vaz Frederic Maxime; De Leonardis Francesco; Fiermonte Giuseppe; Palmieri Ferdinando; Gillissen Bernhard; Daniel Peter T; Jimenez Erin; Walsh Susan; Koehler Carla M; Roy Soumya Sinha; Walter Ludivine; Hajnoczky Gyoergy; Gross Atan^*

来源：Nature Cell Biology, 2010, 12(6): 553-562.

DOI：10.1038/ncb2057

The BH3-only BID (BH3-interacting domain death agonist) protein has a critical function in the death-receptor pathway in the liver by triggering mitochondrial outer membrane permeabilization (MOMP). Here we show that MTCH2/MIMP (mitochondrial carrier homologue 2/Met-induced mitochondrial protein), a novel truncated BID (tBID)-interacting protein, is a surface-exposed outer mitochondrial membrane protein that facilitates the recruitment of tBID to mitochondria. Knockout of MTCH2/MIMP in embryonic stem cells and in mouse embryonic fibroblasts hinders the recruitment of tBID to mitochondria, the activation of Bax/Bak, MOMP, and apoptosis. Moreover, conditional knockout of MTCH2/MIMP in the liver decreases the sensitivity of mice to Fas-induced hepatocellular apoptosis and prevents the recruitment of tBID to liver mitochondria both in vivo and in vitro. In contrast, MTCH2/MIMP deletion had no effect on apoptosis induced by other pro-apoptotic Bcl-2 family members and no detectable effect on the outer membrane lipid composition. These loss-of-function models indicate that MTCH2/MIMP has a critical function in liver apoptosis by regulating the recruitment of tBID to mitochondria.

• 出版日期2010-6