Low melting point pyridinium ionic liquid pretreatment for enhancing enzymatic saccharification of cellulosic biomass

作者：Uju; Nakamoto Aya; Shoda Yasuhiro; Goto Masahiro; Tokuhara Wataru; Noritake Yoshiyuki; Katahira Satoshi; Ishida Nobuhiro; Ogino Chiaki; Kamiya Noriho^*

来源：Bioresource Technology, 2013, 135: 103-108.

DOI：10.1016/j.biortech.2012.06.096

• Biomass
• Cellulose
• Enzymatic saccharification
• Ionic liquid
• Pretreatment

The potential of 1-hexylpyridinium chloride ([Hpy][Cl]), to pretreat cellulosic feedstocks was investigated using microcrystalline cellulose (Avicel) and Bagasse at 80 degrees C or 100 degrees C. Short [Hpy][Cl] pretreatments, %26lt;30 min, at lower temperature accelerate subsequent enzymatic saccharification of Avicel. Over 95% conversion of pretreated Avicel to glucose was attained after 24 h enzymatic saccharification under optimal conditions, whereas regenerated Bagasse showed 1-3-fold higher conversion than untreated biomass. FT-IR analysis of both Avicel and Bagasse samples pretreated with [Hpy][Cl] or 1-ethyl-3-methyimidazolium acetate ([Emim][OAc]) revealed that these ionic liquids behaved differently during pretreatment. [Hpy][Cl] pretreatment for an extended duration (180 min) released mono- and disaccharides without using cellulase enzymes, suggesting [Hpy][Cl] has capability for direct saccharification of cellulosic feedstocks. On the basis of the results obtained, [Hpy][Cl] pretreatment enhanced initial reaction rates in enzymatic saccharification by either crystalline polymorphic alteration of cellulose or partial degradation of the crystalline cellulosic fraction in biomass.

• 出版日期2013-5