Adenovirus-vectored foot-and-mouth disease vaccine confers early and full protection against FMDV O1 Manisa in swine

作者：Fernandez Sainz Ignacio; Medina Gisselle N; Ramirez Medina Elizabeth; Koster Marla J; Grubman Marvin J; de los Santos Teresa^*

来源：Virology, 2017, 502: 123-132.

DOI：10.1016/j.virol.2016.12.021

• FMDV
• Adenovirus
• O1Manisa
• Foot-and-mouth
• Disease
• Cross-protection
• Ad5-FMD
• Vaccine
• Swine

A human adenovirus (Ad5) vectored foot-and-mouth disease virus (FMDV) O1-Manisa subunit vaccine (Ad5-O1Man) was engineered to deliver FMDV O1-Manisa capsid and capsid-processing proteins. Swine inoculated with Ad5-O1Man developed an FMDV-specific humoral response as compared to animals inoculated with an empty Ad5-vector. Vaccinated animals were completely protected against homologous challenge at 7 or 21 days post-vaccination. Potency studies exhibited a PD50 of about 10(7) pfu/animal while a dose of 4x 10(7) pfu/animal fully protected swine against FMDV intradermal challenge. In-vitro cross-neutralization analysis distinctly predicted that swine vaccinated with Ad5-O1Man would be protected against challenge with homologous FMDV O1Man Middle East-South Asia (ME-SA) topotype and also against recent outbreak strains of Mya-98 South East Asia (SEA) lineage including O1-UK-2001 and O1-South Korea-2010. These results indicate that recombinant Ad5-O1Man is an effective, safe and cross-reacting vaccine that could potentially be used preventively and in outbreak situations, to control FMDV O Mya-98 lineage in swine.

• 出版日期2017-2