Arabinoxylo-Oligosaccharides and Inulin Impact Inter-Individual Variation on Microbial Metabolism and Composition, Which Immunomodulates Human Cells

作者：Van den Abbeele Pieter; Taminiau Bernard; Pinheiro Iris; Duy**urgh Cindy; Jacobs Heidi; Pijls Loek; Marzorati Massimo^*

来源：Journal of Agricultural and Food Chemistry, 2018, 66(5): 1121-1130.

DOI：10.1021/acs.jafc.7b04611

• arabinoxylan
• fructan
• gut microbiota
• Caco-2 cells
• In vitro

Fecal batch fermentations coupled to cocultures of epithelial cells and macrophages were used to compare how arabinoxylo-oligosaccharides (AXOS) and inulin modulate gut microbial activity and composition of three different human donors and subsequently the epithelial permeability and immune response. Both inulin and AXOS decreased the pH during incubation (-1.5 pH units), leading to increased productions of acetate, propionate, and butyrate. Differences in terms of metabolites production could be linked to specific microbial alterations at genus level upon inulin/AXOS supplementation (i.e., Bifidobacterium, Bacteroides, Prevotella and unclassified Erysipelotrichaceae), as shown by 16S-targeted Illumina sequencing. Both products stimulated gut barrier and immune function with increases in TEER, NF-KB, IL-10, and IL-6. Ingredients with different structures selectively modulate the microbiota of a specific donor leading to differential changes at metabolic level. The extent of this effect is donor specific and is linked to a final specific modulation of the host's immune system.

• 出版日期2018-2-7