A genetic association study of single nucleotide polymorphisms in GN beta 3 and COMT in elderly patients with irritable bowel syndrome

作者：Wang Yuezhi; Wu Zhengyu; Qiao Hui; Zhang Yu^*

来源：MEDICAL SCIENCE MONITOR, 2014, 20: 1246-1254.

DOI：10.12659/msm.890315

• Dental Care for Elderly
• Single Nucleotide Polymorphisms
• Irritable Bowel Syndrome

Background: Several polymorphisms have been reported to be associated with irritable bowel syndrome (IBS), including C825T, the single nucleotide polymorphism (SNP), responsible for a truncated G protein beta 3 subunit (GN beta 3), and the Vall158Met substitution in catechol-O-methyltransferase (COMT). We investigated the association between these mutations and the prevalence of IBS in 66 elderly Chinese patients.
Material/Methods: Sixty-six patients (over age 60 years) were diagnosed with IBS according to the Rome III criteria, and divided into 3 groups based on symptom presentation. The groups consisted of 7 patients with constipation, 46 patients with diarrhea, and 13 patients with both or neither symptoms. We enrolled 115 age-matched individuals without IBS as the control group. All patients were evaluated by using the Geriatric Depression Scale, disease progression was recorded, and GN beta 3 and COMT were genotyped by PCR.
Results: There was no significant difference in GN beta 3 C825T genotype distribution and allele frequency between the 2 groups. In contrast, compared with control subjects, COMT 158Met was significantly more prevalent in the IBS group (P=0.040) and significantly more prevalent in patients with diarrhea (P=0.029). 158Met was also more prevalent in those patients who had experienced symptoms for over 5 years (P=0.022).
Conclusions: In elderly Chinese patients, the 158Met SNP in COMT is associated with IBS pathogenesis, but the GN beta 3-C825T SNP is not associated with IBS pathogenesis.

• 出版日期2014-7-19
• 单位复旦大学